Discussion on the choice of cleaning solvent for anion exchange chromatography column

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VH10179

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The anion exchange chromatography column is a high-purity silica gel with good mechanical stability, and a quaternary ammonium strong anion exchange group is bonded to the silica gel by using a high-purity bonding reagent, and has a mixed chemical structure of a quaternary ammonium and a phenyl functional group. This mode of mixing the strong anion exchange phase with the hydrophobic phase provides efficient and highly selective separation of various organic compounds such as aromatic or aliphatic carboxylic acids and sulfonic acids. Silicone: spherical, high purity (metal impurities 10ppm)

      The operating pressure of the anion exchange column should not exceed the maximum pressure specified in the column specification, otherwise it will cause irreversible loss of the chromatogram.

      The anion exchange chromatography column is started with a standard eluent (generally referred to as the rinsing conditions) to balance the leaching, and the chromatographic standard anion is tested for separation and importance.

      Storage of the furnace column In general, a standard eluent can be used as a stock solution. For long-term storage, both ends of the column need to be sealed. However, for non-suppressed ion chromatography columns or early suppression ion chromatography columns, since all eluents are relatively neutral, it is easy to grow mold with eluent storage, so it is necessary to use a high pH solution as a stock solution, or Add antifungal agent to the eluent as a stock solution.

      For anion chromatography, there are usually three cleaning methods for cleaning acid-soluble, alkali-soluble or organic contaminants. In the cleaning process, it must be ensured that the cleaning process is carried out in strict accordance with the cleaning process, otherwise it will cause local high pressure of the column and damage the column. For organic solvent cleaning columns, it is necessary to gradually reduce the amount of organic solvent to avoid the viscosity of the mobile phase due to mixing.

      Anion exchange chromatography column cleaning solvent selection for low-cost hydrophilic pollutants: use 10 times the eluent;

      For high-priced hydrophilic pollutants: use 1-3mol/L hydrochloric acid as eluent

      For the metal contaminant: causing chromatographic asymmetry, such as iron ions can cause the sulphate and phosphate tailing, aluminum can cause a decrease in the recovery of the phosphate, a complexing agent acid, such as 0. 2mol / oxalic acid;

      Organic contaminants: Organic contaminants can cause a decrease in column efficiency. They can be cleaned with a compatible organic solvent. Sometimes it can be more effectively mixed with a high concentration of hydrochloric acid and an organic solvent, such as 200mmol/L hydrochloric acid and 80% acetonitrile. It is important to note that the various solutions should be used immediately after mixing. Mixing for a long time will cause acid decomposition and damage the column. Pay special attention to column organic compatibility. If the column is only compatible with 15% organic solvent, the organic solvent concentration should not exceed

15%.

 

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