What are the cleaning methods of chromatographic sample bottles?
What are the cleaning methods of chromatographic sample bottles?
The sampling bottle is the container for the instrument analysis of the material to be analyzed.
This paper summarizes various methods of cleaning chromatographic sample bottles, with the aim of providing a meaningful reference for everyone. These methods have been verified by friends and predecessors, and have a good washing effect on the lipid soluble residue and organic reagent residue in the chromatographic sample bottle. The cleanliness meets the requirements, the cleaning steps are simple, the cleaning time is reduced, and the cleaning process is more environmentally friendly. Please choose your own laboratory according to your own situation!
At present, with the increasing attention to food quality and safety from all walks of life, chromatographic analysis technology has been increasingly applied to food quality and safety detection, especially in the field of agricultural products detection, and chromatographic analysis technology has been widely used. In China, a large number of samples of agricultural products (other chemical products, organic acids, etc.) need to be tested by liquid chromatography and gas chromatography every year.
Due to the large number of samples, a large number of sample bottles need to be cleaned during the test, which not only wastes time and reduces the working efficiency, but also sometimes results in deviation of the test results due to the failure of the cleanliness of the sample bottles after cleaning.
The chromatographic sampling bottle is made of glass, rarely plastic. Disposable sampling bottles are costly, wasteful and seriously polluted to the environment. Most laboratories reuse the bottles after cleaning. At present, the methods commonly used in the laboratory to clean the sample bottles are mainly to add washing powder, detergent, organic solvent and acid and alkali lotion, and then to brush with a small test tube. The conventional cleaning method has many disadvantages, such as large amount of detergent and water, long washing time, easy to leave a dead Angle. If it is plastic into the sample bottle, it is easy to leave a brush mark on the inner bottle wall, which takes up a lot of human resources. For glassware seriously polluted by lipid and protein residues, xie zhenhua et al.  used alkaline cracking solution for cleaning, and achieved good results.
When LC/MS/MS analysis samples, the cleaning of the sample bottle is very important. According to the washing method of glass apparatus, the cleaning method is selected according to the degree of pollution. There is no fixed mode. Method summary:
Solution 1:1. Pour the test solution into the dry bottle. 3. Pour in water and wash with ultrasonic twice. 4. Pour the dry liquid into the bottle and bake at 110 degrees Celsius for 1-2 hours. Never bake at high temperature. 5. Cool and save.
2. Put the beaker filled with pure water (Millipore water machine) and ultrasonic for 15 minutes. 3. Replace the water with ultrasonic for 15 minutes.
Plan 3: first soak with methanol (chromatographic purity) (some waste (primary school), then rinse with ultrasonic for 20 minutes, then pour the methanol out to dry.
Plan 4: (the first), are generally first rinse dry reoccupy after sulfuric acid potassium dichromate lotion soaked (the second), the sample bottle washing method and liquid phase is the same as the other, the first to use medical alcohol covered more than 4 hours, then ultrasonic half an hour, and then poured out medical alcohol, using ultrasonic water half an hour, wash with water after drying.
Solution 5: 1, if the cost is enough, every time with new best 2, if you want to reuse, cleaning method is also very important, first of all, with strong oxidation cleaning fluid (potassium dichromate) for 24 hours, and then use deionized water under the condition of ultrasonic cleaning three times, finally clean with methanol, the drying can be used, and 3, bottle mat must be replaced with new, especially the analysis of pesticide residues, must change, otherwise it will affect the quantitative result. But if the condition permits, or try to use a one-time consumables, For example, the disposable ptfe inner tube or domestic plastic inner tube (0.1 yuan/about) can be used repeatedly without cleaning.
Plan 6: if it is still inclined to carry forward the good style of diligence and frugality, the following Suggestions are suggested: (1) cumbersome cleaning and reliable results: after using the sample bottle, first rinse the sample bottle with flowing water and wash the remaining samples away (at the same time, you can throw them away by hand); No2. Then put the sample bottle into the potassium dichromate washing vacuole, and when it accumulates to a certain amount or when it is in a good mood, remove it from the washing liquid cylinder and put it into a plastic sieve in the kitchen. No3. After washing, ultrasonic cleaning with tap water should be carried out for about 3 times. After each ultrasonic cleaning, it is better to throw out the water in the sample bottle. No4. Wash the product three times with three steamed water (or pure water, deionized water) and 1.3 ultrasonic water; No5. Ultrasonic washing with chromatographic pure methanol for 2-3 times, and the methanol in the sample bottle is also best thrown out after each cleaning; No6. Place the sample bottle in the oven and dry about 80 degrees. (2) buy sample bottles marked in different colors: I wonder if you have noticed that there is a small piece of colored mark on the sample bottle, which is not for the sake of looking good, but has its use significance. When buying, it is best to buy several different colors of bottles. For example: your lab opened at the same time A, B two project, A project using the white sample bottles for the first time, B project use blue sample bottles, after testing in accordance with the above method after the completion of cleaning, the second experiment, A project use blue sample bottles, B project with white sample bottles, and so on, which can effectively avoid the pollution to the trouble of your work.
Finally: 1. Several instrument engineers have suggested that: bake for half an hour at about 400 degrees in a muffle oven. 2. Put the sample bottle into the muffle furnace and dry it at 300 degrees Celsius. An engineer from Beijing, agilent, once said that the world would be clean after 6 hours of baking in the 300-degree muffle furnace. . Small volumetric flask, rotating-evaporating pear-shaped flask and other analytical or pre-treatment glassware can be cleaned with reference to this method.